GHK–Cu 50 mg

Injectable Peptide · Reference / Rev. 01 / 2026

Glycyl-L-Histidyl-L-Lysine : Copper(II) • Subcutaneous

A clinician’s reference for reconstitution, dosing, administration, and cycling of GHK-Cu lyophilized 50 mg vials. For research and educational use.

§ 01

Compound Data Card

CompoundCopper Tripeptide-1

Vial Size50 mg, lyophilized

RouteSubcutaneous (SC)

Half-life≈ 30–60 min

Typical Cycle8–12 weeks (up to 16)

Solution ColorClear, faint blue tint

§ 01

Reconstitution

Use bacteriostatic water (BAC water) for reconstitution.
Inject slowly down the inner wall of the vial — do not spray directly onto the powder.
Roll the vial gently between your palms. Do not shake.
Refrigerate immediately. Use within 30 days.

50 mg ÷ 3.0 mL BAC water = 16.67 mg/mL

Target Dose	Volume	U-100 Units	Notes
0.5 mg	0.03 mL	3 units	Low-sensitivity titration start
1.0 mg	0.06 mL	6 units	Standard daily dose
1.5 mg	0.09 mL	9 units	Intermediate
2.0 mg	0.12 mL	12 units	Upper standard / 3x-week dose

§ 02

Dosing Protocols

ADaily Standard

5 on / 2 off

1–2 mg daily, Monday through Friday. Rest on weekends. Duration: 8–12 weeks.

≈ 5–10 mg / week

BThrice Weekly

Mon / Wed / Fri

2 mg per injection, three times per week. Duration: 8–16 weeks.

≈ 6 mg / week

CPhased Escalation

30 on / 30 off

Days 1–15: 1 mg daily. Days 16–30: 2 mg daily. Then 30 days off.

≈ 7–14 mg / week

§ 03

Administration

Prepare

Wash hands thoroughly. Gather vial, syringe, alcohol swabs, and sharps container.

Swab

Wipe the vial stopper with an alcohol swab. Clean the injection site.

Draw

Invert the vial and withdraw the exact number of units for your target dose.

Inject

Pinch the skin at the site. Insert needle at 45–90°. Inject slowly and steadily.

Rotate

Alternate injection sites across abdomen quadrants to reduce irritation.

Log

Record the date, dose, injection site, and any observations in your log.

§ 04

Storage & Handling

Storage

Lyophilized: Store at -20°C (freezer) for long-term
Reconstituted: Refrigerate at 2–8°C
Shelf life: Use within 30 days after reconstitution

Solution Checks

Solution should be clear with a faint blue tint
Discard if particulates, cloudiness, or discoloration appear
Label the vial with reconstitution date

§ 05

Cycling Logic

Peptide receptors can desensitize with continuous use. Structured on/off cycling preserves signal fidelity, maintains receptor sensitivity, and may sustain long-term efficacy. Most protocols include a rest phase equal to 25–50% of the active phase.

On 8 wk

Off 4 wk

On 8 wk

Off 4 wk

30 on / 30 off
8 wk on / 4 wk off
12 wk on / 4 wk off

§ 06

Safety & Regulatory

Important Notice

GHK-Cu is not FDA-approved for any therapeutic indication. It is supplied for research use only. Any clinical application must be conducted under the supervision of a qualified, licensed healthcare provider. Do not self- administer without professional guidance. This guide does not constitute medical advice.